Interactive In-situ Fish Larvae Observations from Acoustic Enrichment Field Experiments

Autonomous cameras reveal larval reef fish responses to acoustic enrichment and lunar phase, Supplemental Materials, Scientific Reports (2026)

Research Overview

Acoustic enrichment (AE)—the playback of ambient sound from healthy coral reefs—shows promise in attracting fish larvae to degraded or artificial reefs, but previous evaluations have used invasive diver-based sampling techniques, limiting most studies to short deployments in benign and accessible environments. This study used fully autonomous cameras to non-invasively evaluate AE efficacy in attracting settlement-stage fish larvae and mature fish over large fractions of a lunar cycle. We deployed an autonomous AE system in Kāne'ohe Bay (O‘ahu, Hawai‘i) over three spawning events (August 2023, June–July 2024). Treatment (active speaker) and control (inactive speaker) sites were built by placing artificial structures, autonomous cameras, and autonomous hydrophones on a sandy seabed at 5 m water depth, with sites separated by 42-65 m. Treatment and control designations were alternated between deployments to remove potential spatial bias. Manual and semi-automated image analysis found larval counts at both sites peaking around the new moon, but the treatment site attracted 4-14 times more larvae. Both sites encountered similar numbers of mature fish. These results demonstrate that autonomous camera systems can non-invasively study fish larval presence and provide further support that AE can enhance larval fish response.

Acoustic Enrichment Protocol

Listen below to the snapping shrimp, purring damselfish and other reef sounds collected from a local vibrant reef.

Healthy Reef
Vibrant reef (300m away from experiment site) where the AE recording was collected.

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Four-second sample of healthy coral reef sound collected in Kāne'ohe Bay (O‘ahu, Hawai‘i) 300 m from the experimental site during the new moon (August 27, 2022). Hawaiian snapping shrimp (Synalpheus parneomeris) and reef fish, such as the Hawaiian Damselfish (Dascyllus albisella), dominate the ambient sound field.

Open Source Cameras for Autonomous, Remote, Multi-Week Monitoring

Camera facing forward
Cameras were mounted on a 1.27 cm diameter threaded steel rod 33 cm away from a FHM entrance, ensuring consistent distance and coverage for each camera.
Camera side profile
Autonomous and accessible visual monitoring: ESP32-S microcontroller with the OV2640 camera and real time clock ATMEGA325P (Ecologis Consulting, Inc) module encased in a watertight clear acrylic housing with flanged endcaps. The system is powered by two nickel metal hydride battery packs installed in a custom battery mount. All components cost 255.00 USD as of January 2025, with housing accounting for approximately 75 percent of the total cost
August 2023 🌑 New Moon 8/16
June 2024 🌑 New Moon 6/6
July 2024 🌑 New Moon 7/5
Treatment
Control

𓇼Learn more about the science behind the artificial reef structures used in these field experiments here𓇼